The lab visited Hospital Hole in the Weeki Wachee River to obtain samples for Madison. Dr. Garey and Bobby made the dives with Madison in charge of the boat and sampling strategy and sample processing. Hospital Hole is a highly stratified sink located under the Weeki Wachee River near Weeki Wachee Gardens in Florida. It takes about 90 minutes to get there from USF. Our normal boat launch site is Roger's park which was closed for some parking lot resurfacing so we had to go to another park further downstream. Once we launched it took about 30 minutes to reach the site. We hit the water around 1:30 PM and made a series of dives. We collected water from the overlying river, from the hypoxic zone, an active vent in the hypoxic zone, the chemocline, and the deeper anoxic zone. We also collected biofilm from some zones and sediment from the bottom. In total we filled about 50 sample bottles. It was getting dark when we headed back and there was a beautiful sunset along the river. Then the students pulled an all nighter in the lab getting all the samples processed and all the tests run.